Prof. Geiger Tamar

Associate Professor, Department of Human Molecular Genetics and Biochemistry, Sackler Faculty of Medicine, Tel Aviv University

 

Dr. Geiger completed her B.Sc. studies in life sciences at the Hebrew University of Jerusalem. She then continued her M.Sc. and Ph.D. studies at the same university, where she studied cancer signaling under the supervision of Prof. Alexander Levitzki. For her post-doctoral training, she moved to the laboratory of Prof. Matthias Mann at the Max Planck Institute of Biochemistry in Martinsried (near Munich), Germany in 2008. During her post-doc, Geiger specialized in mass spectrometry-based proteomic, developed techniques and applied it to cancer-related questions. In 2011, she returned to Israel and opened her independent research group at Tel Aviv University, which concentrates on clinical cancer proteomics. Geiger is a member of the Israeli Center of Research Excellence (I-CORE) “Gene Regulation in Complex Human Disease” and manages the proteomics hub of the center.

 

Proteomics of Breast Cancer Progression

Our main interest is to understand the mechanisms of breast cancer progression. We are using state-of-the-art mass spectrometry-based proteomics to obtain a system-wide view of the tumor proteins. Analysis of the changes in protein levels and modifications that occur during tumor development is aimed to discover novel regulators of transformation. Combination of the proteomics technology with biochemical and genetic methods will show the significance of these candidates to cancer development and may suggest novel drug targets and tumor markers.

 

Pozniak, Y., Lahat, N., Rudolph, J.D., Katzir, R., Avivi, C., Ruppin, E., Barchack, I. & Geiger, T*. Systemwide clinical proteomics of breast cancer reveals global remodeling of cellular homeostasis. Cell Systems, 2:172-84 (2016). *Corresponding author.

 

Tyanova, S., Albrechsten, R., Kronqvist, P., Cox, J.*, Mann, M.* & Geiger, T.* Quantitative clinical proteomics reveals functional maps of breast cancer
subtypes. Nat Communications 7:10259 (2016). *Corresponding author

 

Iglesias-Gato, D., Wikstrom, P., Tyanova, S., Svensson, C., Thysell, E., Carlsson, J., Hägglöf, C., Cox, J, Andren, O., Stattin, P., Egevad, L., Widmark, A., Jartell, A., Collins, C., Bergh, A., Geiger, T., Mann, M. & Flores-Morales, A. The proteome of prostate cancer. European Urology pii: S0302-2838(15)01087-8 (2015).

 

Furth, N., Bossel Ben-Moshe, N., Pozniak, Y., Porat, Z., Geiger, T., Domany, E., Aylon, Y. & Oren, M. Downregulation of LATS kinases alters p53 to promote cell migration. Genes Dev 29 (22): 2325-2330 (2015).

 

Aviner, R., Shenoy, A., Elroy-Stein, O.* & Geiger, T.* (2015). Uncovering hidden layers of cell cycle regulation through integrative multi-omic analysis. PLoS Genetics 11, e1005554. *Corresponding author.

 

Darr, J. Geiger, T., Gordon, J., Isacc, S. & Eden, A. Phosphoprotemic Analysis of Snf5 Deficient Tumor Cells Reveals Activation of EGFr Signaling Which Is Dependent Upon Snf5 Expression. Cancer Genetics 207, 446 (2015).

 

Elbaz-Alon, Y., Eisenberg-Bord, M., Shinder, V., Stiller, S. B., Shimoni, E., Wiedemann, N., Geiger, T. & Schuldiner, M. Lam6 regulates the extent of contacts between organelles. Cell reports 12, 7-14 (2015).

 

Gat‐Viks, I., Geiger, T., Barbi, M., Raini, G., & Elroy‐Stein, O. Proteomics‐level analysis of myelin formation and regeneration in a mouse model for Vanishing White Matter disease. J Neurochemistry 134, 513-526 (2015).

 

Shkedy, D., Singh, N., Shemesh, K., Amir, A., Geiger, T., Liefshitz, B., Harari, Y. & Kupiec, M. Regulation of Elg1 activity by phosphorylation. Cell Cycle 15 (2015).

 

Nathan, G., Kredo-Russo, S., Geiger, T., Lenz, A., Kaspi, H., Hornstein, E., & Efrat, S. MiR-375 Promotes Redifferentiation of Adult Human β Cells Expanded In Vitro. PloS One 10, e0122108 (2015).

 

Shenoy, A. & Geiger, T*. Super-SILAC: Current trends and future perspectives. Expert Reviews of Proteomics 12, 13-19 (2015). *Corresponding author.

 

Lossos, A., Elazar, N., Lerer, I., Schueler-Furman, O., Fellig, Y., Glick, B., Zimmerman, B.E. Azulay, H., Dotan, S., Goldberg, S., Gomori, J.M., Ponger, P., Newman, J.P., Marreed, H., Steck, A.J., Schaeren-Wiemers, N., Mor, N., Harel, M., Geiger, T., Eshed-Eisenbach, Y., Meiner, V., & Peles, E. Myelinassociated glycoprotein gene mutation causes Pelizaeus-Merzbacher disease-like disorder. Brain 138, 2521-2536 (2015).

 

Harel, M., Oren-Giladi, P., Kaidar-Person, O., Shaked, Y., & Geiger, T.* Proteomics of Microparticles with SILAC Quantification (PROMIS-Quan): A Novel Proteomic Method for Plasma Biomarker Quantification. Mol Cell Proteomics 14, 1127-1136 (2015). *Corresponding author.

 

Salpeter, S. J., Pozniak, Y., Merquiol, E., Ben-Nun, Y., Geiger, T., & Blum, G. A novel cysteine cathepsin inhibitor yields macrophage cell death and mammary tumor regression. Oncogene, Mar 23 (2015).

 

Sharon, Y., Raz, Y., Cohen, N., Ben-Shmuel, A., Schwartz, H., Geiger, T., & Erez, N. Tumor-derived Osteopontin reprograms normal mammary fibroblasts to promote inflammation and tumor growth in breast cancer. Cancer research 75, 963-973 (2015).

 

Biran, A., Perelmutter, M., Gal, H., Burton, D. G., Ovadya, Y., Vadai, E., Geiger, T. & Krizhanovsky, V. Senescent cells communicate via intercellular protein transfer. Genes Dev 29, 791-802 (2015).

 

Karaköse, E., Geiger, T., Flynn, K., Lorenz-Baath, K., Zent, R., Mann, M., & Fässler, R. The focal adhesion protein PINCH-1 associates with EPLIN at integrin adhesion sites. J Cell Science 128, 1023-1033 (2015).

 

Rivlin, N., Katz, S., Doody, M., Sheffer, M., Horesh, S., Molchadsky, A., Koifman, G., Shetzer, Y., Goldfinger, N., Rotter, V. and Geiger, T. Rescue of ESCs from cellular transformation by proteomic stabilization of mutant p53 and conversion into WT conformation. Proc Natl Acad Sci USA, 111, 7006-11 (2014).

 

Oren, Y.S., McClure, M.L., Rowe, S.M., Sorscher, E.J., Bester, A.C., Manor, M., Kerem, E., Rivlin, J., Mann, M., Geiger, T. and Kerem, B. The Unfolded Protein Response affects readthrough of Premature Termination Codons. EMBO Molec Med. 6, 685-701 (2014).

 

Pozniak, Y. and Geiger, T. Design and application of super-SILAC for proteome quantification. Meth Molec Biol. 1188, 281-291 (2014).

 

Aviner, R., Geiger, T.* and Elroy-Stein, O. Genome-wide identification and quantification of protein synthesis in cultured cells and whole tissues by puromycin-associated nascent chain proteomics (PUNCH-P). Nat Protocols 9, 751-760 (2014). * Single corresponding author.

 

Aviner, R., Geiger, T. and Elroy-Stein, O. PUNCH-P for global translatome profiling; Methodology, insights and comparison to other techniques. Translation 1, e27516 (2014).

 

Waldman, Y.Y., Geiger, T. and Ruppin, E. A genome-wide systematic analysis reveals different and predictive proliferation expression signatures of cancerous vs. non-cancerous cells. PLoS Gen. 9, e1003806 (2013).

 

Aviner, R., Geiger, T*. and Elroy-Stein, O*. Novel proteomic approach (PUNCH-P) reveals cell cycle-specific fluctuations in mRNA translation. Genes and Dev 27:1834-44 (2013). * Equal contribution corresponding authors.

 

Radovanac, K., Morgner, J., Schulz, J.N., Blumbach, K., Patterson, C., Geiger, T., Mann, M., Krieg, T., Eckes, B., Fässler, R. and Wickström, S.A. Stabilization of integrin-linked kinase by the Hsp90-CHIP axis impacts cellular force generation, migration and the fibrotic response. EMBO J. 32(10):1409-24 (2013)

 

Geiger, T., Velic, A., Macek, B., Lundberg, E., Kampf, C., Nagaraj, N., Uhlen, M., Cox, J. and Mann, M. Initial quantitative proteomic map of 28 mouse tissues using the SILAC mouse. Mol Cell Proteomics 12:1709-22 (2013)

 

Boersema, P.J., Geiger, T., Wisniewski, J.R. and Mann, M. Quantification of the N-glycosylated secretome by super-SILAC during breast cancer progression and in human blood samples. Mol Cell Proteomics 12:158-71 (2013)

 

Jerby, L., Wolf, L., Denkert, C., Stein, G.Y., Hilvo, M., Oresic, M., Geiger, T.* and Ruppin E*. Metabolic associations of reduced proliferation and oxidative stress in advanced breast cancer. Cancer Res. 72(22):5712-20 (2012). Corresponding authors.

 

Geiger, T., Madden, S.F., Gallagher, W.M., Cox, J. and Mann M. Proteomic portrait of human breast cancer progression identifies novel prognostic markers. Cancer Res. 72:2428-2439 (2012)

 

Schaab, C., Geige, T., Stoehr, G., Cox, J. and Mann, M. Analysis of high accuracy, quantitative proteomics data in the MaxQB database. Mol Cell Proteomics. 11:M111.014068 (2012)

 

Geiger, T, Wehner, A., Schaab, C., Cox, J. and Mann, M. Comparative proteomic analysis of eleven common cell lines reveals ubiquitous but varying expression of most proteins. Mol Cell Proteomics. 11:M111.014050 (2012)

 

Nagaraj, N., Wisniewski, J.R., Geiger, T., Cox, J., Kircher, M., Kelso, J., Paabo, S. & Mann, M. Deep proteome and transcriptome mapping of a human cancer cell line. Mol Syst Biol. 7:548 (2011).

 

Meves, A., Geiger, T., Zanivan, S., DiGiovanni, J., Mann, M. & Fässler, R. β1 integrin cytoplasmic tyrosines promote tumorigenesis in a phosphorylation-independent manner. Proc Natl Acad Sci USA 108:15213-15218 (2011)

 

Thakur, S.*, Geiger, T.*, Chatterjee, B., Bandilla, P., Fröhlich, F., Cox, J. & Mann, M. Deep proteome coverage in single-run liquid chromatography tandem mass spectrometry.  Mol Cell Proteomics 10:M110.003699 (2011) * Equal contribution.

 

Geiger, T., Wisniewski, JR., Cox, J., Zanivan, S., Kruger, M., Ishihama, Y. & Mann, M. Use of stable isotope labeling by amino acids in cell culture (SILAC) as an internal standard in quantitative proteomics. Nat Protocols 6:147-157 (2011).

 

Lundberg, E., Fagerberg, L., Matic, I., Geiger, T., Cox, J., Klevebring, D., Älgenäs, C., Lundeberg, J., Mann, M & Uhlen, M. Highly ubiquitous expression of the transcriptome and proteome in three functionally different human cell lines. Mol Syst Biol 6:450 (2010).

 

Geiger, T., Cox, J., Ostasiewicz, P., Wisniewski, J.R. & Mann, M. Super-SILAC mix for quantitative proteomics of human tumor tissue. Nat Methods 7:383-385 (2010).

 

Geiger, T., Cox, J. & Mann, M. Proteomics on an Orbitrap benchtop mass spectrometer using all-ion fragmentation. Mol Cell Proteomics 9:2252-2261 (2010).

 

Geiger, T., Cox, J. & Mann, M. Proteomic changes resulting from gene copy number variations in cancer cells. PLoS Genet 6:e1001090 (2010).

 

 

Reviews

Shenoy, A. & Geiger, T*. Super-SILAC: Current trends and future perspectives. Expert Reviews of Proteomics 12, 13-19 (2015). *Corresponding author.

 

Geiger T*, Zaidel-Bar R*. Opening the floodgates: proteomics and the integrin adhesome. Curr Opin Cell Biol. 24(5):562-8 (2012).* Equal contribution corresponding authors.

 

• 2012-2017 Israeli Center for Research Excellence (I-CORE): Gene Regulation in Complex Human Disease
• 2012-2017 Israel Science Foundation (ISF) Grant: The role of metabolic pathways in the regulation of breast cancer progression.
• 2015-2020 European Research Council- ERC starting grant: Topoproteomic profiling of breast cancer heterogeneity
• 2014-2017 Israel Ministry of Science and Technology: Proteogenomic analysis of breast cancer