Molecular analysis of the human cancer cell with special emphasis on membrane proteins, tumor-associated antigens, ligand-receptor interactions and cell signaling. We work on a breast-cancer associated protein designated MUC1 (CA-15-3) that is a membrane protein participating in cell signaling.
Pichinuk E, Benhar I, Jacobi O, Chalik M, Weiss L, Ziv R, Sympson C, Karwa A, Smorodinsky NI, Rubinstein DB, Wreschner DH. Antibody targeting of cell-bound MUC1 SEA domain kills tumor cells. Cancer Res. 2012 Jul 1;72(13):3324-36. doi: 10.1158/0008-5472.CAN-12-0067. Epub 2012 Apr 16. PubMed PMID: 22507854.
Margalit M, Yogev L, Yavetz H, Lehavi O, Hauser R, Botchan A, Barda S, Levitin F, Weiss M, Pastan I, Wreschner DH, Paz G, Kleiman SE. Involvement of the prostate and testis expression (PATE)-like proteins in sperm-oocyte interaction. Hum Reprod. 2012 May;27(5):1238-48. doi: 10.1093/humrep/des064. Epub 2012 Mar 8. PubMed PMID: 22402205; PubMed Central PMCID: PMC3329192.
Pichinuk E, Broday L, Wreschner DH. Endogenous RNA cleavages at the ribosomal SRL site likely reflect miRNA (miR) mediated translational suppression. Biochem Biophys Res Commun. 2011 Nov 4;414(4):706-11. doi: 10.1016/j.bbrc.2011.09.140. Epub 2011 Oct 6. PubMed PMID: 22001924.
Pichinuk E, Wreschner DH. Similarities between Argonautes and the alpha-sarcin-like ribotoxins: Implications for microRNA action. Protein Sci. 2010 Jun;19(6):1272-8. doi: 10.1002/pro.391. PubMed PMID: 20512980; PubMed Central PMCID: PMC2895252.
Horn G, Gaziel A, Wreschner DH, Smorodinsky NI, Ehrlich M. ERK and PI3K regulate different aspects of the epithelial to mesenchymal transition of mammary tumor cells induced by truncated MUC1. Exp Cell Res. 2009 May 1;315(8):1490-504. doi: 10.1016/j.yexcr.2009.02.011. Epub 2009 Feb 23. PubMed PMID: 19245809.
Levitin F, Weiss M, Hahn Y, Stern O, Papke RL, Matusik R, Nandana SR, Ziv R, Pichinuk E, Salame S, Bera T, Vincent J, Lee B, Pastan I, Wreschner DH. PATE gene clusters code for multiple, secreted TFP/Ly-6/uPAR proteins that are expressed in reproductive and neuron-rich tissues and possess neuromodulatory activity. J Biol Chem. 2008 Jun 13;283(24):16928-39. doi: 10.1074/jbc.M801454200. Epub 2008 Apr 3. PubMed PMID: 18387948; PubMed Central PMCID: PMC2423272.
Rubinstein DB, Karmely M, Pichinuk E, Ziv R, Benhar I, Leitner O, Ningpeng F, Smorodinsky NI and Wreschner DH. The MUC1 oncoprotein as a functional target: Immunotoxin binding to / junction mediates cell killing. Int. J. Cancer 2008 (In Press).
Zaretsky JZ and Wreschner DH. Protein multifunctionality: Principles and mechanisms. Translational Oncogenomics (2008) In Press
Levitin F, Weiss M, Hahn Y, Stern O, Papke RL, Matusik R, Nandana SR, Ziv R, Pichinuk E, Bera T, Vincent J, Lee B, Pastan I, and Wreschner DH. Gene Clusters Code for Multiple, Secreted, TFP/Ly-6/uPAR Proteins That Are Expressed in Reproductive and Neural Tissues and Possess Neuromodulatory Activity. J Biol Chem. 283, 2008 (16928-16939).
Rubinstein, D.B., Karmely,M., Ziv, R., Benhar, I., Leitner, O., Baron, S., Katz, B-Z., and Wreschner, D.H. MUC1/X protein immunization enhances cDNA immunization in generating anti-MUC1 a/b junction antibodies that target malignant cells. Cancer Res. 66, 2006 (11247-11253).
Zaretsky, J.Z., Barnea, I., Aylon, Y., Gorivodsky, M., Wreschner, D.H., and Keydar, I. MUC1 SEA gene overexpressed in breast cancer: Structure and transcriptional activity of the MUC1 promoter and role of estrogen receptor alpha (ERa) in regulation of the MUC1 gene expression. Molecular Cancer 5, 2006 (pp57-71).
Levitin F, Stern O, Weiss M, Gil-Henn C, Ziv R, Prokocimer Z, Smorodinsky NI, Rubinstein DB and Wreschner DH. The MUC1 SEA module is a self-cleaving domain. J Biol Chem. 280, 2005 (33374-33386).
Levitin, F., Baruch, A., Weiss, M., Steigman, K., Hartman, M., Yoeli-Lerner, M.
A., Ziv, R., Zrihan-Licht, S., Sheena, S., Gat, A., Lifschitz, B., Simha, M., Stadler, Y., Cholostoy, A., Gil, B., Greaves, D.R., Keydar, I., Smorodinsky, N., and Wreschner, D.H. A novel protein isoform derived from the MUC1 gene by alternative splicing and frameshifting. J. Biol. Chem. 280, 2005 (pp10655-10663).
Hey NA, Meseguer M, Simon C, Smorodinsky NI, Wreschner DH, Ortiz ME, Aplin JD. Transmembrane and truncated (SEC) isoforms of MUC1 in the human endometrium and Fallopian tube. Reprod Biol Endocrinol. 2003 Jan 30;1(1):2.
Wreschner DH, McGuckin MA, Williams SJ, Baruch A, Yoeli M, Ziv R, Okun L, Zaretsky J, Smorodinsky N, Keydar I, Neophytou P, Stacey M, Lin HH, Gordon S. Generation of ligand-receptor alliances by "SEA" module-mediated cleavage of membrane-associated mucin proteins. Protein Sci. 2002 Mar;11(3):698-706.
Williams SJ, Wreschner DH, Tran M, Eyre HJ, Sutherland GR, McGuckin MA. Muc13, a novel human cell surface mucin expressed by epithelial and hemopoietic cells. J Biol Chem. 2001 May 25;276(21):18327-36.
Baruch A, Hartmann M, Yoeli M, Adereth Y, Greenstein S, Stadler Y, Skornik Y, Zaretsky J, Smorodinsky NI, Keydar I, Wreschner DH. The breast cancer-associated MUC1 gene generates both a receptor and its cognate binding protein. Cancer Res. 1999 Apr 1;59(7):1552-61.
Hartman M, Baruch A, Ron I, Aderet Y, Yoeli M, Sagi-Assif O, Greenstein S, Stadler Y, Weiss M, Harness E, Yaakubovits M, Keydar I, Smorodinsky NI, Wreschner DH. MUC1 isoform specific monoclonal antibody 6E6/2 detects preferential expression of the novel MUC1/Y protein in breast and ovarian cancer. Int J Cancer. 1999 Jul 19;82(2):256-67.
Baruch A, Hartmann M, Zrihan-Licht S, Greenstein S, Burstein M, Keydar I, Weiss M, Smorodinsky N, Wreschner DH. Preferential expression of novel MUC1 tumor antigen isoforms in human epithelial tumors and their tumor-potentiating function. Int J Cancer. 1997 May 29;71(5):741-9.
Zrihan-Licht S, Baruch A, Elroy-Stein O, Keydar I, Wreschner DH. Tyrosine phosphorylation of the MUC1 breast cancer membrane proteins. Cytokine receptor-like molecules. FEBS Lett. 1994 Dec 12;356(1):130-6.
Zrihan-Licht S, Vos HL, Baruch A, Elroy-Stein O, Sagiv D, Keydar I, Hilkens J, Wreschner DH. Characterization and molecular cloning of a novel MUC1 protein, devoid of tandem repeats, expressed in human breast cancer tissue. Eur J Biochem. 1994 Sep 1;224(2):787-95.
Wreschner DH, Hareuveni M, Tsarfaty I, Smorodinsky N, Horev J, Zaretsky J, Kotkes P, Weiss M, Lathe R, Dion A, and I. Keydar. Human epithelial tumor antigen cDNA sequences. Differential splicing may generate multiple protein forms. Eur J Biochem. 1990 May 20;189(3):463-73.
Weiss M, Baruch A, Keydar I, Wreschner DH. Preoperative diagnosis of thyroid papillary carcinoma by reverse transcriptase polymerase chain reaction of the MUC1 gene. Int J Cancer. 1996 Mar 28;66(1):55-9.
Wreschner DH, McCauley JW, Skehel JJ, Kerr IM. Interferon action--sequence specificity of the ppp(A2'p)nA-dependent ribonuclease. Nature. 1981 Jan 29;289(5796):414-7.
Knight M, Cayley PJ, Silverman RH, Wreschner DH, Gilbert CS, Brown RE, Kerr IM. Radioimmune, radiobinding and HPLC analysis of 2-5A and related oligonucleotides from intact cells. Nature. 1980 Nov 13;288(5787):189-92.
